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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using CHST4 Monoclonal antibody (66623-1-Ig)

    WB analysis of HT-29 using 66623-1-Ig (same clone as 66623-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using CHST4 Monoclonal antibody (66623-1-Ig)

    WB analysis of T-47D using 66623-1-Ig (same clone as 66623-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CHST4 Monoclonal antibody (66623-1-Ig)

    WB analysis of MCF-7 using 66623-1-Ig (same clone as 66623-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CHST4 Monoclonal antibody (66623-1-Ig)

    WB analysis of COLO 320 using 66623-1-Ig (same clone as 66623-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHST4 Monoclonal antibody (66623-1-Ig)

    IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHST4 Monoclonal antibody (66623-1-Ig)

    IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHST4 Monoclonal antibody (66623-1-Ig)

    IF Staining of HeLa using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CHST4 antibody (66623-1-Ig, Clone: 1E1A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHST4 Monoclonal antibody (66623-1-Ig)

    IF Staining of HeLa using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66623-1-Ig (CHST4 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CHST4 Monoclonal antibody, PBS Only

CHST4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66623-1-PBS
Clone No.1E1A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Gn6st-2, Gn6st 2, GlcNAc6ST-2, GlcNAc6ST 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66623-1-PBS targets CHST4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHST4 fusion protein Ag3987 相同性解析による交差性が予測される生物種
Full Name carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC035282
Gene Symbol CHST4
Gene ID (NCBI) 10164
RRIDAB_2881983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NCG5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)