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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CIN85 Monoclonal antibody, PBS Only

CIN85 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12D2

Cat no : 66571-1-PBS

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis using 66571-1-Ig (same clone as 66571-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of K-562 using 66571-1-Ig (same clone as 66571-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of PC-12 using 66571-1-Ig (same clone as 66571-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of rat brain using 66571-1-Ig (same clone as 66571-1-PBS)

    rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66571-1-PBS targets CIN85 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CIN85 fusion protein Ag2777 相同性解析による交差性が予測される生物種
Full Name SH3-domain kinase binding protein 1
Calculated molecular weight 665 aa, 73 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC015806
Gene symbol SH3KBP1/CIN85
Gene ID (NCBI) 30011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.