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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CIN85 Monoclonal antibody

CIN85 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C12D2

Cat no : 66571-1-Ig

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis using 66571-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of K-562 cells using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of K-562 using 66571-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of PC-12 using 66571-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CIN85 Monoclonal antibody (66571-1-Ig)

    WB analysis of rat brain using 66571-1-Ig

    rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHL-60 cells, rat brain tissue, K-562 cells, PC-12 cells, mouse brain tissue, THP-1 cells, Jurkat cells, Ramos cells, Raji cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66571-1-Ig targets CIN85 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CIN85 fusion protein Ag2777 相同性解析による交差性が予測される生物種
Full Name SH3-domain kinase binding protein 1
Calculated molecular weight 665 aa, 73 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC015806
Gene symbol SH3KBP1/CIN85
Gene ID (NCBI) 30011
RRIDAB_2881932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Protocols

Product Specific Protocols
WB protocol for CIN85 antibody 66571-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols