Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, THP-1 cells, HL-60 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66571 targets CIN85 in WB applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CIN85 fusion protein Ag2777 相同性解析による交差性が予測される生物種 |
| Full Name | SH3-domain kinase binding protein 1 |
| Calculated molecular weight | 665 aa, 73 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC015806 |
| Gene Symbol | SH3KBP1/CIN85 |
| Gene ID (NCBI) | 30011 |
| RRID | AB_3084260 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96B97 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 CIN85 antibody CL488-66571 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |