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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated CISD1 Polyclonal ant (CL647-16006)

    FC experiment of HeLa using CL647-16006

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human CISD1 (CL647-16006) and HRP-conjugated polymer at dilution 1:1000 (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CISD1 Polyclonal antibody

CISD1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-16006

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

CISD1, Cysteine transaminase CISD1, EC:2.6.1.3, MDS029, MitoNEET, ZCD1, C10orf70, CDGSH iron sulfur domain 1, CDGSH iron-sulfur domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-16006 targets CISD1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CISD1 fusion protein Ag8680 相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 1
Calculated molecular weight 108 aa, 12 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
RRIDAB_2934922
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ45
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CISD1 antibody CL647-16006Download protocol
Standard Protocols
Click here to view our Standard Protocols