Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue |
| Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
66082-1-Ig targets CISD2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.
| Tested Reactivity | human, zebrafish, rat, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | CDGSH iron sulfur domain 2 |
| Observed molecular weight | 15 kDa |
| GenBank accession number | NM_001008388 |
| Gene Symbol | CISD2 |
| Gene ID (NCBI) | 493856 |
| RRID | AB_11232425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N5K1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CISD2-Specific antibody 66082-1-Ig | Download protocol |
| IHC protocol for CISD2-Specific antibody 66082-1-Ig | Download protocol |
| IF protocol for CISD2-Specific antibody 66082-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Commun Biol Integrating apaQTL and eQTL analysis identifies a potential causal variant associated with lung adenocarcinoma risk in the Chinese population | ||
J Biomed Sci Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis | ||
Mol Neurodegener Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin models | ||
Nat Commun ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome |