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  • Featured Product
  • KD/KO Validated

CISD2-Specific Monoclonal antibody, PBS Only

CISD2-Specific Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D7A3

Cat no : 66082-1-PBS

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

    WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of ROS1728 using 66082-1-Ig (same clone as 66082-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of RAW 264.7 using 66082-1-Ig (same clone as 66082-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    WB analysis of zebrafish using 66082-1-Ig (same clone as 66082-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    IHC staining of human testis using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    IHC staining of human testis using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CISD2-Specific Monoclonal antibody (66082-1-Ig)

    IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66082-1-PBS targets CISD2-Specific in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.

Tested Reactivity human, zebrafish, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 2
Observed molecular weight 15 kDa
GenBank accession numberNM_001008388
Gene symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.