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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 647-conjugated CITED1 Polyclonal an (CL647-26999)

    FC experiment of HEK-293 using CL647-26999

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CITED1 (CL647-26999) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-26999

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-26999 targets CITED1 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CITED1 fusion protein Ag24643 相同性解析による交差性が予測される生物種
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
RRIDAB_2934965
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99966
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CITED1 antibody CL647-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols