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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16686

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CKAP4 Polyclonal antibody (CL594-16686)

    IF Staining of HepG2 using CL594-16686

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CKAP4 antibody (CL594-16686) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated CKAP4 Polyclonal antibody (CL594-16686)

    FC experiment of HeLa using CL594-16686

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CKAP4 (CL594-16686) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-16686 targets CKAP4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP4 fusion protein Ag10019 相同性解析による交差性が予測される生物種
Full Name cytoskeleton-associated protein 4
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC082972
Gene symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_2919837
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Protocols

Product Specific Protocols
IF protocol for CL594 CKAP4 antibody CL594-16686Download protocol
FC protocol for CL594 CKAP4 antibody CL594-16686Download protocol
Standard Protocols
Click here to view our Standard Protocols