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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CKMT1A Recombinant antibody, PBS Only

CKMT1A Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240825A4

Cat no : 83750-4-PBS

Synonyms

CKMT1B, CKMT1, CKMT, CKMiMi, Acidic-type mitochondrial creatine kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKMT1A Recombinant antibody (83750-4-RR)

    WB analysis using 83750-4-RR (same clone as 83750-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83750-4-RR (CKMT1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83750-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83750-4-RR

    Affinity and Kinetic Characterization of 83750-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83750-4-RR against Human CKMT1A were performed. The affinity constant is 2.86 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83750-4-PBS is CKMT1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKMT1A fusion protein Ag7583 相同性解析による交差性が予測される生物種
Full Name creatine kinase, mitochondrial 1A
Calculated molecular weight 47 kDa
Observed molecular weight45 kDa
GenBank accession numberBC001926
Gene symbol CKMT1A
Gene ID (NCBI) 548596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.