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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CKMT1A Recombinant antibody

CKMT1A Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240825A4

Cat no : 83750-4-RR

Synonyms

CKMT1B, CKMT1, CKMT, CKMiMi, Acidic-type mitochondrial creatine kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKMT1A Recombinant antibody (83750-4-RR)

    WB analysis using 83750-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83750-4-RR (CKMT1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83750-4-RR

    Affinity and Kinetic Characterization of 83750-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83750-4-RR against Human CKMT1A were performed. The affinity constant is 2.86 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, SH-SY5Y cells, mouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83750-4-RR is CKMT1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKMT1A fusion protein Ag7583 相同性解析による交差性が予測される生物種
Full Name creatine kinase, mitochondrial 1A
Calculated molecular weight 47 kDa
Observed molecular weight45 kDa
GenBank accession numberBC001926
Gene symbol CKMT1A
Gene ID (NCBI) 548596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for CKMT1A antibody 83750-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols