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- KD/KO Validated
CLIC4 Monoclonal antibody
CLIC4 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B7F9
Cat no : 66343-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HuH-7 cells, pig heart tissue, HeLa cells, U2Os cells, pig kidney tissue, rabbit kidney tissue, rat kidney tissue, mouse kidney tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
66343-1-Ig targets CLIC4 in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CLIC4 fusion protein Ag2942 相同性解析による交差性が予測される生物種 |
| Full Name | chloride intracellular channel 4 |
| Calculated molecular weight | 253 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC012444 |
| Gene symbol | CLIC4 |
| Gene ID (NCBI) | 25932 |
| RRID | AB_2881723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLIC4 antibody 66343-1-Ig | Download protocol |
| IHC protocol for CLIC4 antibody 66343-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. |
