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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis using 66343-1-Ig (same clone as 66343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis of pig heart using 66343-1-Ig (same clone as 66343-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human kidney using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human kidney using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLIC4 Monoclonal antibody (66343-1-Ig)

    IF Staining of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIC4 antibody (66343-1-Ig, Clone: 1B7F9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of BT-549 cells using CLIC4 Monoclonal antibody (66343-1-Ig)

    FC experiment of BT-549 using 66343-1-Ig (same clone as 66343-1-PBS)

    1x10^6 BT-549 cells were intracellularly stained with 0.2 μg CLIC4 Monoclonal antibody (66343-1-Ig, Clone: 1B7F9) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CLIC4 Monoclonal antibody, PBS Only

CLIC4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66343-1-PBS
Clone No.1B7F9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MTCLIC, huH1, H1, FLJ38640, CLIC4L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66343-1-PBS targets CLIC4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLIC4 fusion protein Ag2942 相同性解析による交差性が予測される生物種
Full Name chloride intracellular channel 4
Calculated molecular weight 253 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC012444
Gene Symbol CLIC4
Gene ID (NCBI) 25932
RRIDAB_2881723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y696
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.