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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLSTN2 Polyclonal antibody (21436-1-AP)

    WB analysis using 21436-1-AP (same clone as 21436-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21436-1-AP (CLSTN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21436-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLSTN2 Polyclonal antibody (21436-1-AP)

    IHC staining of mouse brain using 21436-1-AP (same clone as 21436-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21436-1-AP (CLSTN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21436-1-PBS in a different storage buffer formulation.

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CLSTN2 Polyclonal antibody, PBS Only

CLSTN2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21436-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21436-1-PBS targets CLSTN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLSTN2 fusion protein Ag16048 相同性解析による交差性が予測される生物種
Full Name calsyntenin 2
Calculated molecular weight 955 aa, 107 kDa
Observed molecular weight100-107 kDa
GenBank accession numberBC104485
Gene Symbol CLSTN2
Gene ID (NCBI) 64084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4D0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CLSTN2 gene encodes the synaptic protein calsyntenin 2, one of the three calsyntenin family members found in humans. CLSTN2 is expressed exclusively in the brain, with high levels occurring in cortical gamma-aminobutyric acid (GABA) interneurons and in medial temporal lobe regions (PMID: 19058786). Western blot analysis detected CLSTN2 at an apparent molecular mass of 107 kDa.