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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLTCL1 Monoclonal antibody

CLTCL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5A10

Cat no : 66274-1-Ig

Synonyms

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of HeLa using 66274-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of HEK-293 using 66274-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of Jurkat using 66274-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IHC staining of human skeletal muscle using 66274-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IHC staining of human skeletal muscle using 66274-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IF Staining of HeLa using 66274-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66274-1-Ig targets CLTCL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name clathrin, heavy chain-like 1
Calculated molecular weight 187 kDa
Observed molecular weight 180 kDa
GenBank accession numberNM_007098
Gene symbol CLTCL1
Gene ID (NCBI) 8218
RRIDAB_2881657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.

Protocols

Product Specific Protocols
WB protocol for CLTCL1 antibody 66274-1-IgDownload protocol
IHC protocol for CLTCL1 antibody 66274-1-IgDownload protocol
IF protocol for CLTCL1 antibody 66274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols