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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of HEK-293 using 66274-1-Ig (same clone as 66274-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    WB analysis of Jurkat using 66274-1-Ig (same clone as 66274-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTCL1 Monoclonal antibody (66274-1-Ig)

    IF Staining of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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CLTCL1 Monoclonal antibody, PBS Only

CLTCL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66274-1-PBS
Clone No.1B5A10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66274-1-PBS targets CLTCL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name clathrin, heavy chain-like 1
Calculated molecular weight 187 kDa
Observed molecular weight 180 kDa
GenBank accession numberNM_007098
Gene Symbol CLTCL1
Gene ID (NCBI) 8218
RRIDAB_2881657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53675
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.