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Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 12289-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12289-1-AP targets Clusterin in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Clusterin fusion protein Ag2889 相同性解析による交差性が予測される生物種
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_2083316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocols

Product Specific Protocols
WB protocol for Clusterin antibody 12289-1-APDownload protocol
IHC protocol for Clusterin antibody 12289-1-APDownload protocol
IF protocol for Clusterin antibody 12289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Clin Invest

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Authors - Xiaochao Tan
humanWB

Proc Natl Acad Sci U S A

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Authors - Lei Shi

Sci Signal

Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release.

Authors - Lili Donner
WB

Front Cell Dev Biol

Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.

Authors - Xinlei Li
humanWB

Ecotoxicol Environ Saf

Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.

Authors - Yu Ma
IHC

Food Chem Toxicol

Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.

Authors - Carmen Rodríguez-Rivera
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