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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12289

Synonyms

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-12289

    IF Staining of HeLa using CL488-12289

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Clusterin antibody (CL488-12289) at dilution of 1:100, CL594-phalloidin (red).

  • IF Staining of human liver using CL488-12289

    IF Staining of human liver using CL488-12289

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Clusterin antibody (CL488-12289) at dilution of 1:100.

  • IF Staining of human liver using CL488-12289

    IF Staining of human liver using CL488-12289

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Clusterin antibody (CL488-12289) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells, human liver tissue
For other applications, we recommend the unconjugated version of this antibody, 12289-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-12289 targets Clusterin in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Clusterin fusion protein Ag2889 相同性解析による交差性が予測される生物種
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC010514
Gene symbol CLU
Gene ID (NCBI) 1191
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biological fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Clusterin antibody CL488-12289Download protocol
Standard Protocols
Click here to view our Standard Protocols