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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMIP Recombinant antibody, PBS Only

CMIP Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240059C12

Cat no : 83381-1-PBS

Synonyms

Tc Mip, c-Mip, C-Maf-inducing protein, c Mip, c Maf inducing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CMIP Recombinant antibody (83381-1-RR)

    WB analysis of human peripheral blood platelets using 83381-1-RR (same clone as 83381-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83381-1-RR (CMIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Recombinant antibody (83381-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR (same clone as 83381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Recombinant antibody (83381-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR (same clone as 83381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83381-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using CMIP Recombinant antibody (83381-1-RR)

    FC experiment of A549 using 83381-1-RR (same clone as 83381-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug CMIP Recombinant Antibody (83381-1-RR, Clone:240059C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83381-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83381-1-PBS is CMIP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CMIP fusion protein Ag3475 相同性解析による交差性が予測される生物種
Full Name c-Maf-inducing protein
Calculated molecular weight 86 kDa
Observed molecular weight69 kDa
GenBank accession numberBC038113
Gene symbol CMIP
Gene ID (NCBI) 80790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.