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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CMIP Recombinant antibody (83381-1-RR)

    WB analysis of human peripheral blood platelets using 83381-1-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83381-1-RR (CMIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Recombinant antibody (83381-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Recombinant antibody (83381-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using CMIP Recombinant antibody (83381-1-RR)

    FC experiment of A549 using 83381-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug CMIP Recombinant Antibody (83381-1-RR, Clone:240059C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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CMIP Recombinant antibody

CMIP Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83381-1-RR
Clone No.240059C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Tc Mip, c-Mip, C-Maf-inducing protein, c Mip, c Maf inducing protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83381-1-RR targets CMIP in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CMIP fusion protein Ag3475 相同性解析による交差性が予測される生物種
Full Name c-Maf-inducing protein
Calculated molecular weight 86 kDa
Observed molecular weight69 kDa
GenBank accession numberBC038113
Gene Symbol CMIP
Gene ID (NCBI) 80790
RRIDAB_3671035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.

Protocols

Product Specific Protocols
WB protocol for CMIP antibody 83381-1-RRDownload protocol
IHC protocol for CMIP antibody 83381-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols