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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using CNNM4 Recombinant antibody (83824-5-RR)

    IF Staining of LNCaP using 83824-5-RR (same clone as 83824-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83824-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CNNM4 Recombinant antibody (83824-5-RR)

    IF Staining of MCF-7 using 83824-5-RR (same clone as 83824-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83824-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83824-5-RR

    Affinity and Kinetic Characterization of 83824-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83824-5-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

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CNNM4 Recombinant antibody, PBS Only

CNNM4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83824-5-PBS
Clone No.240873C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83824-5-PBS targets CNNM4 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CNNM4 fusion protein Ag5121 相同性解析による交差性が予測される生物種
Full Name cyclin M4
Calculated molecular weight 87 kDa
GenBank accession numberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6P4Q7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).