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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using CNNM4 Recombinant antibody (83824-5-RR)

    IF Staining of LNCaP using 83824-5-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CNNM4 Recombinant antibody (83824-5-RR)

    IF Staining of MCF-7 using 83824-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83824-5-RR

    Affinity and Kinetic Characterization of 83824-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83824-5-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

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CNNM4 Recombinant antibody

CNNM4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83824-5-RR
Clone No.240873C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inLNCaP cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83824-5-RR targets CNNM4 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CNNM4 fusion protein Ag5121 相同性解析による交差性が予測される生物種
Full Name cyclin M4
Calculated molecular weight 87 kDa
GenBank accession numberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
RRIDAB_3671409
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6P4Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

Protocols

Product Specific Protocols
IF protocol for CNNM4 antibody 83824-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols