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- KD/KO Validated
CNOT1 Monoclonal antibody
CNOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1A10A11
Cat no : 66507-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, A549 cells, HEK-293 cells, human cerebellum tissue, pig kidney tissue, mouse kidney tissue, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
66507-1-Ig targets CNOT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CNOT1 fusion protein Ag5623 相同性解析による交差性が予測される生物種 |
| Full Name | CCR4-NOT transcription complex, subunit 1 |
| Calculated molecular weight | 267 kDa |
| Observed molecular weight | 240-250 kDa |
| GenBank accession number | BC040523 |
| Gene symbol | CNOT1 |
| Gene ID (NCBI) | 23019 |
| RRID | AB_2881870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| IHC protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| IF protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.
| ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aditya (Verified Customer) (11-01-2024) | It was alright, seems to be good for hrp but not the best with flourescence
|
FH Roy (Verified Customer) (06-12-2024) | Great antibody to detect CNOT1 by WB from HeLa nuclear and cytoplasmic extracts (1/1000 - Overnight incubation at 4°C). Does also detect the cytoplasmic CNOT1 by immunofluorescence (1/250) in PFA 4% fixed HeLa cells.
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