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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

    WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis of mouse kidney using 66507-1-Ig (same clone as 66507-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis of HEK-293 using 66507-1-Ig (same clone as 66507-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human cerebellum tissue using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis of human cerebellum using 66507-1-Ig (same clone as 66507-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using CNOT1 Monoclonal antibody (66507-1-Ig)

    WB analysis of pig kidney using 66507-1-Ig (same clone as 66507-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CNOT1 Monoclonal antibody (66507-1-Ig)

    IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CNOT1 Monoclonal antibody (66507-1-Ig)

    IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CNOT1 Monoclonal antibody (66507-1-Ig)

    IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CNOT1 Monoclonal antibody, PBS Only

CNOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66507-1-PBS
Clone No.1A10A11

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66507-1-PBS targets CNOT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC040523
Gene Symbol CNOT1
Gene ID (NCBI) 23019
RRIDAB_2881870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA5YKK6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.