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  • Featured Product
  • KD/KO Validated

CNOT8 Polyclonal antibody, PBS Only

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10752-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of Jurkat using 10752-1-AP (same clone as 10752-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IP experiment of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    IF Staining of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10752-1-AP (CNOT8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10752-1-PBS targets CNOT8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT8 fusion protein Ag1201 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 8
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017366
Gene symbol CNOT8
Gene ID (NCBI) 9337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.