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CNPase Monoclonal antibody

CNPase Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B9G9

Cat no : 66729-1-Ig

Synonyms

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis using 66729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of HepG2 using 66729-1-Ig

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells.

  • Western Blot (WB) analysis of U-251 cells using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of U-251 using 66729-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of human brain using 66729-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CNPase Monoclonal antibody (66729-1-Ig)

    IF Staining of mouse brain using 66729-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using CNPase Monoclonal antibody (66729-1-Ig)

    FC experiment of HepG2 using 66729-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inpig cerebellum tissue, U-251 cells, human brain tissue, HepG2 cells, rat brain tissue, rat cerebellum tissue
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66729-1-Ig targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNPase fusion protein Ag4227 相同性解析による交差性が予測される生物種
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight 421 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC028040
Gene symbol CNPase
Gene ID (NCBI) 1267
RRIDAB_2882079
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Protocols

Product Specific Protocols
WB protocol for CNPase antibody 66729-1-IgDownload protocol
IF protocol for CNPase antibody 66729-1-IgDownload protocol
FC protocol for CNPase antibody 66729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

CNS Neurosci Ther

EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury

Authors - Yingmei Zhang
View Article
ratWB

J Ethnopharmacol

Shenzhiling oral liquid protects the myelin sheath against Alzheimer's disease through the PI3K/Akt-mTOR pathway.

Authors - Mingcui Zheng
View Article