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  • Featured Product
  • KD/KO Validated

CNPase Monoclonal antibody, PBS Only

CNPase Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B9G9

Cat no : 66729-1-PBS

Synonyms

CNP, CNP1, CNPase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of U-251 using 66729-1-Ig (same clone as 66729-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CNPase Monoclonal antibody (66729-1-Ig)

    WB analysis of human brain using 66729-1-Ig (same clone as 66729-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CNPase Monoclonal antibody (66729-1-Ig)

    IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66729-1-PBS targets CNPase in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig, mouse samples.

Tested Reactivity Human, Rat, Pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNPase fusion protein Ag4227 相同性解析による交差性が予測される生物種
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight 421 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC028040
Gene symbol CNP
Gene ID (NCBI) 1267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.