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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNTFR Polyclonal antibody, PBS Only

CNTFR Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10796-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis using 10796-1-AP (same clone as 10796-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of human brain using 10796-1-AP (same clone as 10796-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of mouse cerebellum using 10796-1-AP (same clone as 10796-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of SH-SY5Y using 10796-1-AP (same clone as 10796-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of human heart using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of human heart using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10796-1-PBS targets CNTFR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTFR fusion protein Ag1189 相同性解析による交差性が予測される生物種
Full Name ciliary neurotrophic factor receptor
Calculated molecular weight 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC001492
Gene symbol CNTFR
Gene ID (NCBI) 1271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.