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COASY Monoclonal antibody

COASY Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 68358-1-Ig
Clone No.1D1C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68358-1-Ig targets COASY in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COASY fusion protein Ag33142 相同性解析による交差性が予測される生物種
Full Name Coenzyme A synthase
Calculated molecular weight 564 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC020985
Gene Symbol COASY
Gene ID (NCBI) 80347
RRIDAB_3085080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13057
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.

Protocols

Product Specific Protocols
WB protocol for COASY antibody 68358-1-IgDownload protocol
IHC protocol for COASY antibody 68358-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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