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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COASY Monoclonal antibody (68358-1-Ig)

    WB analysis using 68358-1-Ig (same clone as 68358-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68358-1-Ig (COASY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using COASY Monoclonal antibody (68358-1-Ig)

    IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using COASY Monoclonal antibody (68358-1-Ig)

    IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COASY Monoclonal antibody (68358-1-Ig)

    FC experiment of HeLa using 68358-1-Ig (same clone as 68358-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug COASY Monoclonal antibody (68358-1-Ig, Clone:1D1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

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COASY Monoclonal antibody, PBS Only

COASY Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 68358-1-PBS
Clone No.1D1C11

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Synonyms

Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68358-1-PBS targets COASY in Indirect ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COASY fusion protein Ag33142 相同性解析による交差性が予測される生物種
Full Name Coenzyme A synthase
Calculated molecular weight 564 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC020985
Gene Symbol COASY
Gene ID (NCBI) 80347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13057
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.