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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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Collagen Type XVII Polyclonal antibody, PBS Only

Collagen Type XVII Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31190-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31190-1-PBS targets Collagen Type XVII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type XVII fusion protein Ag34645 相同性解析による交差性が予測される生物種
Full Name collagen, type XVII, alpha 1
Observed molecular weight180 kDa
GenBank accession numberBC168368
Gene Symbol COL17A1
Gene ID (NCBI) 1308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UMD9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).