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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type I Recombinant antibody, PBS Only (Capture)

Collagen Type I Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240665D2

Cat no : 83752-3-PBS

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Collagen Type I Recombinant antibody (83752-3-RR)

    WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Collagen Type I Recombinant antibody (83752-3-RR)

    WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1, COL1A2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-1-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2, COL1A2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-2-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83752-3-RR

    Affinity and Kinetic Characterization of 83752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83752-3-PBS targets Collagen Type I as part of a matched antibody pair:

MP00716-1: 83752-3-PBS capture and 83752-1-PBS detection (validated in Cytometric bead array)

MP00716-2: 83752-3-PBS capture and 83752-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight100-160 kDa
GenBank accession numberBC054498
Gene symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.