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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type I Recombinant antibody

Collagen Type I Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240665D2

Cat no : 83752-3-RR

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Collagen Type I Recombinant antibody (83752-3-RR)

    WB analysis of rat skin using 83752-3-RR

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Collagen Type I Recombinant antibody (83752-3-RR)

    WB analysis using 83752-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83752-3-RR

    Affinity and Kinetic Characterization of 83752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

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Tested Applications

Positive WB detected inMG-63 cells, rat skin tissue, Saos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83752-3-RR is Collagen Type I Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight100-160 kDa
GenBank accession numberBC054498
Gene symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

Protocols

Product Specific Protocols
WB protocol for Collagen Type I antibody 83752-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols