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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin tissue using Collagen Type I Recombinant antibody (83752-5-RR)

    IHC staining of human skin using 83752-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using Collagen Type I Recombinant antibody (83752-5-RR)

    IHC staining of human skin using 83752-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HSC-T6 cells using COL1A2 Recombinant antibody (83752-5-RR)

    IF Staining of HSC-T6 using 83752-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HSC-T6 cells using Collagen Type I Recombinant antibody (83752-5-RR)

    IF Staining of HSC-T6 using 83752-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using Collagen Type I antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HSC-T6 cells using Collagen Type I Recombinant antibody (83752-5-RR)

    FC experiment of HSC-T6 using 83752-5-RR

    1x10^6 HSC-T6 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SW480 cells using Collagen Type I Recombinant antibody (83752-5-RR)

    FC experiment of SW480 using 83752-5-RR

    1x10^6 SW480 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83752-5-RR

    Affinity and Kinetic Characterization of 83752-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-5-RR against Human COL1A2 were performed. The affinity constant is 6.77 nM.

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Collagen Type I Recombinant antibody

Collagen Type I Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83752-5-RR
Clone No.240665F8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHSC-T6 cells
Positive FC (Intra) detected inSW480 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83752-5-RR targets Collagen Type I in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
RRIDAB_3671349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08123
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type I antibody 83752-5-RRDownload protocol
IF protocol for Collagen Type I antibody 83752-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols