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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HSC-T6 cells using CoraLite® Plus 488-conjugated Collagen Type I Reco (CL488-83752-5)

    IF Staining of HSC-T6 using CL488-83752-5

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using CoraLite® Plus 488 Collagen Type I antibody (CL488-83752-5, Clone: 240665F8 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated Collagen Type I Recombinant antibody

Collagen Type I Recombinant Antibody for IF/ICC
Cat No. CL488-83752-5
Clone No.240665F8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHSC-T6 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83752-5 targets Collagen Type I in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08123
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Collagen Type I antibody CL488-83752-5Download protocol
Standard Protocols
Click here to view our Standard Protocols