Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis of mouse skin using 22734-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skin using 22734-1-AP
rat skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skin using 22734-1-AP
IP result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:500) with mouse skin tissue lysate 3200ug.
IHC staining of human hepatocirrhosis using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).
IHC staining of human hepatocirrhosis using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).
IHC staining of human malignant melanoma using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).
IHC staining of human skin using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).
IHC staining of human pancreas cancer using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).
IHC staining of human colon using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).
IHC staining of human skin cancer using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 22734-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 22734-1-AP
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human colon using 22734-1-AP
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human colon using 22734-1-AP
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red).
IF Staining of mouse colon using 22734-1-AP
Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).
×
Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

Featured Product
KD/KO Validated

Collagen Type III (N-terminal) Polyclonal antibody

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 22734-1-AP

Publications(497)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

COL3A1, COL3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

HRP

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (497)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skin tissue, rat skin tissue
Positive IP detected in	mouse skin tissue
Positive IHC detected in	human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, mouse kidney tissue, human colon tissue, human skin cancer tissue, mouse heart tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human colon tissue
Positive IF-Fro detected in	mouse colon tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 355 publications below
IHC	See 147 publications below
IF	See 81 publications below

Product Information

22734-1-AP targets Collagen Type III (N-terminal) in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, chicken, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Collagen Type III (N-terminal) fusion protein Ag18658 相同性解析による交差性が予測される生物種
Full Name	collagen, type III, alpha 1
Calculated molecular weight	1466 aa, 139 kDa
Observed molecular weight	140-180 kDa
GenBank accession number	BC028178
Gene Symbol	COL3A1
Gene ID (NCBI)	1281
RRID	AB_2879158
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02461
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Protocols

Product Specific Protocols
WB protocol for Collagen Type III (N-terminal) antibody 22734-1-AP	Download protocol
IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-AP	Download protocol
IF protocol for Collagen Type III (N-terminal) antibody 22734-1-AP	Download protocol
IP protocol for Collagen Type III (N-terminal) antibody 22734-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Metab Sphingolipid metabolism controls mammalian heart regeneration Authors - Xiaoqian Ji View Article
mouse	IHC	Acta Pharm Sin B SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation. Authors - Fen Long View Article
		Sci Adv Designer cellular spheroids with DNA origami for drug screening Authors - Jiayi Wei View Article
	WB,IHC	Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation Authors - Jie Sun View Article
mouse,human	WB	Basic Res Cardiol SAIL: a new conserved anti-fibrotic lncRNA in the heart. Authors - Shenjian Luo View Article
mouse	WB	J Exp Med EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model. Authors - Xixi Tao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skin using 22734-1-AP

WB analysis of rat skin using 22734-1-AP

IP experiment of mouse skin using 22734-1-AP

IHC staining of human hepatocirrhosis using 22734-1-AP

IHC staining of human hepatocirrhosis using 22734-1-AP

IHC staining of human malignant melanoma using 22734-1-AP

IHC staining of human malignant melanoma using 22734-1-AP

IHC staining of mouse colon using 22734-1-AP

IHC staining of mouse colon using 22734-1-AP

IHC staining of mouse heart using 22734-1-AP

IHC staining of mouse heart using 22734-1-AP

IHC staining of human skin using 22734-1-AP

IHC staining of human skin using 22734-1-AP

IHC staining of human pancreas cancer using 22734-1-AP

IHC staining of human pancreas cancer using 22734-1-AP

IHC staining of mouse liver using 22734-1-AP

IHC staining of mouse liver using 22734-1-AP

IHC staining of mouse colon using 22734-1-AP

IHC staining of mouse colon using 22734-1-AP

IHC staining of mouse kidney using 22734-1-AP

IHC staining of mouse kidney using 22734-1-AP

IHC staining of human colon using 22734-1-AP

IHC staining of human colon using 22734-1-AP

IHC staining of human skin cancer using 22734-1-AP

IHC staining of human skin cancer using 22734-1-AP

IF Staining of human colon using 22734-1-AP

IF Staining of human colon using 22734-1-AP

IF Staining of human colon using 22734-1-AP

IF Staining of mouse colon using 22734-1-AP

Collagen Type III (N-terminal) Polyclonal antibody

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Sphingolipid metabolism controls mammalian heart regeneration

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Designer cellular spheroids with DNA origami for drug screening

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

SAIL: a new conserved anti-fibrotic lncRNA in the heart.

EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.