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HRP-conjugated Collagen Type III (N-terminal) Polyclonal antibody

Collagen Type III (N-terminal) Polyclonal Antibody for WB
Cat No. HRP-22734

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size/Concentration: 

-/ -


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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inrat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

HRP-22734 targets Collagen Type III (N-terminal) in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type III (N-terminal) fusion protein Ag18658 相同性解析による交差性が予測される生物種
Full Name collagen, type III, alpha 1
Calculated molecular weight 1466 aa, 139 kDa
Observed molecular weight 140-180 kDa
GenBank accession numberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
RRIDAB_2920509
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02461
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Protocols

Product Specific Protocols
WB protocol for HRP Collagen Type III (N-terminal) antibody HRP-22734Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Burn Care Res

Effects of nanoparticle-mediated dihydromyricelin to diabetic wounds: An in vivo study

Authors - Zhao-Nan Wang
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