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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3H10B1

Cat no : 66824-1-Ig

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI, collagen, type VI, alpha 1, OPLL

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    WB analysis of human skeletal muscle using 66824-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    WB analysis of human heart using 66824-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human liver cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human liver cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human colon cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human colon cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66824-1-Ig targets Collagen Type VI in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag11809 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC052575
Gene symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_2882167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Collagen Type VI antibody 66824-1-IgDownload protocol
IHC protocol for Collagen Type VI antibody 66824-1-IgDownload protocol
IF protocol for Collagen Type VI antibody 66824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols