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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type VI Monoclonal antibody, PBS Only

Collagen Type VI Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3H10B1

Cat no : 66824-1-PBS

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI, collagen, type VI, alpha 1, OPLL

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    WB analysis of human skeletal muscle using 66824-1-Ig (same clone as 66824-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    WB analysis of human heart using 66824-1-Ig (same clone as 66824-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human colon cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IHC staining of human colon cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Collagen Type VI Monoclonal antibody (66824-1-Ig)

    IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66824-1-PBS targets Collagen Type VI in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag11809 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC052575
Gene symbol COL6A1
Gene ID (NCBI) 1291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.