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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using COL6A3-Specific Recombinant antibody (83010-1-RR)

    WB analysis of A375 using 83010-1-RR (same clone as 83010-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COL6A3-Specific Recombinant antibody (83010-1-RR)

    FC experiment of HeLa using 83010-1-RR (same clone as 83010-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00027-3

    Cytometric bead array standard curve of MP00027-3

    Cytometric bead array standard curve of MP00027-3, COL6A3-Specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83010-1-PBS. Detection antibody: 83010-4-PBS. Standard: Ag34752. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83010-1-RR

    Affinity and Kinetic Characterization of 83010-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.

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COL6A3-Specific Recombinant antibody, PBS Only (Capture)

COL6A3-Specific Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83010-1-PBS
Clone No.230540A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

COL6A3, Collagen Type VI, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain, 230540A11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83010-1-PBS targets COL6A3-Specific as part of a matched antibody pair:

MP00027-3: 83010-1-PBS capture and 83010-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COL6A3-Specific fusion protein Ag34752 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 3
Calculated molecular weight 344 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12111
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.