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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using COL6A3-Specific Recombinant antibody (83010-1-RR)

    WB analysis of A375 using 83010-1-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using COL6A3-Specific Recombinant antibody (83010-1-RR)

    FC experiment of HeLa using 83010-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83010-1-RR

    Affinity and Kinetic Characterization of 83010-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.

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COL6A3-Specific Recombinant antibody

COL6A3-Specific Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83010-1-RR
Clone No.230540A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL6A3, Collagen Type VI, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain, 230540A11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83010-1-RR targets COL6A3-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COL6A3-Specific fusion protein Ag34752 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 3
Calculated molecular weight 344 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
RRIDAB_3670754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

Protocols

Product Specific Protocols
WB protocol for COL6A3-Specific antibody 83010-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols