- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated COMMD1 Polyclonal antibody
COMMD1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11938
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11938 targets COMMD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COMMD1 fusion protein Ag2535 相同性解析による交差性が予測される生物種 |
| Full Name | copper metabolism (Murr1) domain containing 1 |
| Calculated molecular weight | 190 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC022046 |
| Gene symbol | COMMD1 |
| Gene ID (NCBI) | 150684 |
| RRID | AB_2919045 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen Affinity Purified |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 COMMD1 antibody CL488-11938 | Download protocol |
| FC protocol for CL Plus 488 COMMD1 antibody CL488-11938 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
