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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated COMMD1 Monoclonal antibody

COMMD1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10E10

Cat no : CL488-67016

Synonyms

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5, 3B10E10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 Monoclonal an (CL488-67016)

    IF Staining of HepG2 using CL488-67016

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 antibody (CL488-67016, Clone: 3B10E10 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 Monoclonal an (CL488-67016)

    FC experiment of HepG2 using CL488-67016

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated COMMD1 Monoclonal antibody (CL488-67016, Clone:3B10E10)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67016 targets COMMD1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag28596 相同性解析による交差性が予測される生物種
Full Name copper metabolism (Murr1) domain containing 1
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COMMD1 antibody CL488-67016Download protocol
Standard Protocols
Click here to view our Standard Protocols