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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COMT Monoclonal antibody

COMT Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 68181-1-Ig

Synonyms

catechol O methyltransferase, COMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMT Monoclonal antibody (68181-1-Ig)

    WB analysis using 68181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using COMT Monoclonal antibody (68181-1-Ig)

    IF Staining of mouse cerebellum using 68181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue
Positive IF detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68181-1-Ig targets COMT in WB, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMT fusion protein Ag6502 相同性解析による交差性が予測される生物種
Full Name catechol-O-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000419
Gene symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Protocols

Product Specific Protocols
WB protocol for COMT antibody 68181-1-IgDownload protocol
IF protocol for COMT antibody 68181-1-IgDownload protocol
FC protocol for COMT antibody 68181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols