• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COMT Monoclonal antibody (68181-1-Ig)

    WB analysis using 68181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using COMT Monoclonal antibody (68181-1-Ig)

    IF Staining of mouse cerebellum using 68181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］論文掲載5,600報突破！GFP免疫沈降用抗体「GFP-Trap®」を無料体験

COMT Monoclonal antibody

COMT Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68181-1-Ig
Clone No.1A1A4

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IF-P, ELISA

Synonyms

catechol O methyltransferase, COMT

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68181-1-Ig targets COMT in WB, IF-P, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMT fusion protein Ag6502 相同性解析による交差性が予測される生物種
Full Name catechol-O-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000419
Gene Symbol COMT
Gene ID (NCBI) 1312
RRIDAB_2935272
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21964
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Protocols

Product Specific Protocols
WB protocol for COMT antibody 68181-1-IgDownload protocol
IF protocol for COMT antibody 68181-1-IgDownload protocol
FC protocol for COMT antibody 68181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols