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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COMT Monoclonal antibody, PBS Only

COMT Monoclonal Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 68181-1-PBS

Synonyms

catechol O methyltransferase, COMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMT Monoclonal antibody (68181-1-Ig)

    WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using COMT Monoclonal antibody (68181-1-Ig)

    IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using COMT Monoclonal antibody (68181-1-Ig)

    FC experiment of HepG2 using 68181-1-Ig (same clone as 68181-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human COMT (68181-1-Ig, Clone:1A1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68181-1-PBS targets COMT in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMT fusion protein Ag6502 相同性解析による交差性が予測される生物種
Full Name catechol-O-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000419
Gene symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.