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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated COPA Recombinant antibody

COPA Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230361B1

Cat no : CL488-83089-2

Synonyms

Alpha coat protein, Alpha COP, Coatomer subunit alpha, COPA, FLJ26320, HEP COP, HEPCOP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Hela cells using CoraLite® Plus 488-conjugated COPA Recombinant ant (CL488-83089-2)

    IF Staining of Hela using CL488-83089-2

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 COPA antibody (CL488-83089-2, Clone: 230361B1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated COPA Recombinant ant (CL488-83089-2)

    FC experiment of HeLa using CL488-83089-2

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated COPA Recombinant antibody (CL488-83089-2, Clone:230361B1)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83089-2 is COPA Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPA fusion protein Ag34207 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit alpha
Calculated molecular weight138 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_004371
Gene symbol COPA
Gene ID (NCBI) 1314
RRIDAB_3673185
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COPA antibody CL488-83089-2Download protocol
FC protocol for CL Plus 488 COPA antibody CL488-83089-2Download protocol
Standard Protocols
Click here to view our Standard Protocols