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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPE Monoclonal antibody (66994-1-Ig)

    WB analysis using 66994-1-Ig (same clone as 66994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COPE Monoclonal antibody (66994-1-Ig)

    IF Staining of HEK-293 using 66994-1-Ig (same clone as 66994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66994-1-PBS in a different storage buffer formulation.

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COPE Monoclonal antibody, PBS Only

COPE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66994-1-PBS
Clone No.2B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66994-1-PBS targets COPE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COPE fusion protein Ag28602 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit epsilon
Calculated molecular weight 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_2882311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14579
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.