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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPE Monoclonal antibody

COPE Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8E8

Cat no : 66994-1-Ig

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPE Monoclonal antibody (66994-1-Ig)

    WB analysis using 66994-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COPE Monoclonal antibody (66994-1-Ig)

    IF Staining of HEK-293 using 66994-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, LNCaP cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66994-1-Ig targets COPE in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COPE fusion protein Ag28602 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit epsilon
Calculated molecular weight 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017285
Gene symbol COPE
Gene ID (NCBI) 11316
RRIDAB_2882311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPE antibody 66994-1-IgDownload protocol
IF protocol for COPE antibody 66994-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols