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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated COPE Monoclonal antibody (CL594-66994)

    WB analysis using CL594-66994

    HeLa cells were subjected to SDS PAGE followed by western blot with CL594-66994 (COPE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CoraLite®594-conjugated COPE Monoclonal antibody

COPE Monoclonal Antibody for WB
Cat No. CL594-66994
Clone No.2B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66994 targets COPE in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COPE fusion protein Ag28602 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit epsilon
Calculated molecular weight 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_2934754
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14579
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CL594 COPE antibody CL594-66994Download protocol
Standard Protocols
Click here to view our Standard Protocols