CoraLite®594-conjugated COPS2 Monoclonal antibody
COPS2 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2H10E6
Cat no : CL594-66435
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human ovary cancer cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66435 targets COPS2 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | COPS2 fusion protein Ag1415 相同性解析による交差性が予測される生物種 |
| Full Name | COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC012629 |
| Gene symbol | COPS2 |
| Gene ID (NCBI) | 9318 |
| RRID | AB_2919990 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 COPS2 antibody CL594-66435 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
